SERVICE & PRODUCT

Liquid Biopsy Equipment

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Cell Isolator CIS030

Smart Biopsy™ Cell Isolator - CIS 030 Smart Biopsy™ Cell Isolator enriches intact rare cells from human blood and/or body fluid using HDM chip.
(High density microporous chip)
- By size-based filtration, it captures viable cells, which can be useful for downstream application including genomic analysis, immunofluorescent staining, and culture.
- It is applicable for all kind of body fluid containing rare cells, e.g., blood, pleural effusion, etc.
- Typical example of enriched rare cells is CTC (circulating tumor cells).

Feature

  • Capture Intact and Pure Cells

    Enrich viable cells with high purity by size-based filtration.

  • Automatically detects liquid layer

    Detect the volume of the liquid layer by camera and process automatically.

  • Utilize Validated CTC Analysis Workflows

    Analyze enriched cells with the downstream assay methods including genomic analysis and immunofluorescent staining.

  • Unique Barcode System

    Detect barcode on sample container to avoid intermixing of samples.

  • Variable Volume Sample Loading

    Process sample loading in 5~10ml

In equipment Workflow

  • STEP 1

    Double Negative Selection - Removal of most of erythrocytes and leukocytes in the whole blood

  • STEP 2

    HDM Chip - Further removal of remaining smaller nucleated cells by size-based gravity flow filtration

  • STEP 3

    CTC Recovery - Retrieval of CTCs

Applications

  • Immunofluorescence Staining

    Images of captured CTCs from patients. CTCs were identified by the following
    criteria : DAPI (+)/EpCAM (+)/ CD45 (-)

  • FISH

    FISH analysis for the detection of ALK rearrangement
    in CTCs from lung cancer patient. Separate red and green signals (arrows)
    and isolated red signals (arrow heads) indicates ALK rearrangement.

  • CTC Culture

    Representative images of cultured CTCs at day 0, 4, 7 and 16.

  • Mutation Analysis in CTC

    EGFR exon 19 deletion test with CTCs from lung cancer patient.

IF Stainer CST030

SmartBiopsy™ IF Stainer - CST 030 SmartBiopsy™ IF Stainer is fully automated immunofluorescent staining system, which can stain cells
on the slide with , CD45, anti-EpCAM, CK etc.
- The staining procedure is done in a dark chamber with temperature and moisture control. The system has refrigerated chamber, where reagents are kept.
- Various staining procedures can be done with various antibodies, and up to 12 slides can processed at one time.

Feature & Performance

  • Accurate volume of reagents

    Can release accurate volume of reagents at each step of procedure, and remove reagents on the slides.

  • Automatic mounting

    Mount coverslip automatically with DAPI-containing mounting medium.

  • Cold Storage

    Refrigerated chamber can maintain the temperature of reagents during the procedure.

  • Temperature control chamber

    Automatic temperature & humidity control. External light blocking device.

  • Multiple Sample Loading

    Can process 1~12 slides.

  • Covering Module

    Automatic cover glass attachment device on the stained sample slide.

  • Applications

    Cell staining-based High Throughput screening Development of Staining Protocol.

In equipment Workflow

  • STEP 1

    Liquid Vision

  • STEP 2

    Liquid Dispensing & Incubation

  • STEP 3

    Sample Sealing on Cover glass

Immunofluorescent staining - CIKF10

Immunofluorescent staining image of circulating tumor cells detected from clinical samples.

A

Immunofluorescent staining for circulating tumor cell specific biomarkers and leukocyte expression proteins was
performed to identify circulating cancer cells isolated from various cancers.

B

4-channel analysis using two different cancer cell-specific biomarkers (EpCAM & CK) is possible using circulating
tumor cells from patients with colorectal cancer.

Cell Image Analyzer CIA040

Smart Biopsy™ Cell Image Analyzer - CIA 040 The Smart Biopsy™ Cell Image Analyzer captures immunofluorescent images of cells stained with
anti-EpCAM, -CD45, -CK antibodies. The system includes Image Analyzing software.
- Cell image capture on accurate location provides high-quality merged image of multi-fluorescence.
- The software provides individual values of intensity and morphology of each cell. Entire slide image and/or magnified image can be obtained.
- All modules can be operated by both automatic and manual control.

Feature & Performance

  • Single Slide Loading

    Single slide loading with automatic cover for opening and closing

  • Multiple Color Shot on Same Position

    High-quality of merged image.

  • Automatic Analysis

    Automatic Sample Image Extraction.

  • Easy-Operating Software

    Individual value of fluorescent intensity, cell morphology of each cell. Real time zoom in/out of images on the slide.

Feature & Performance

  • Fluorescence Intensity Analysis

    - Analysis of target protein analysis based on immunofluorescence staining

    - Analysis of cell morphology

  • Automatic Target Counting

    - Automatic counting capacity : more than ten thousands of cells

    - Automation : From analysis to Data report

  • Cancer Cell Counting

    - Filter mode : For detection of specific cancer cells.

In equipment Workflow

  • STEP 1

    Sample Loading on platform

  • STEP 2

    Focusing & Shooting

  • STEP 3

    Analyzing & Reporting